"PhilGeneStrips Biosensor Kit for Quick Detection of Shrimp Pathogen "

Agency: Southeast Asian Fisheries Development Center- Aquaculture Department

Partner Institution: Ateneo de Manila University & University of the Philippines Diliman

Rationale:

PhilGeneStrips Biosensor Kit for Quick Detection of Shrimp Pathogen, can help the Farmers to easily detect if the shrimp have a Disease, awareness of emerging disease on shrimp and it is affordable to the  farmers because it can’t cost to much money to buy this PhilGeneStrips  and can used in mobile by searching  application. In  Shrimp Farm Cloud Monitoring Mobile App. It can reads test strips result, includes strips info (QR code) for quality assurance and sends data to shrimp Farm Cloud monitoring website or database so it can easily monitor pond status for Shrimp Pathogens and manage your shrimp farm using this application. In our generation we found out that the technologies was appeared in our society,Even in the Past generation they have advance technologies to share and to say something about themselves  so the farmers and other people with Bussiness can use this Application to Prevent spreading of disease to other Sea Creature.

Shrimp Pathogen Background:

This Online Phillipine Shrimp Pathogen Information resource (OPSPIR) is an online database of shrimp pathogen recorded in the country. It includes isolate reports from outbreaks and sampling of various pathogens including White Spot Syndrome Virus (WSSV), Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV), Larval Virus (LV), and Acute Hepatopancreatic Necrosis Disease (AHPND). Aside from the online database, a biobank was Established containing shrimp tissue, isolated bacteria and viruses or their DNA or RNA stored archived with physical barcoding in biosecure ultralow freezers. OPSPIR will be made accesible to the public through a website and an online server to increase stakeholders’ knowledge of the pathogens and to accelarate the development of strategies for their management and control. 

Methods/ Procedure

Using the test kit:

1.       Put shrimp tissue inside tube. Add lysis buffer Homogenize tissue using pestle.

2.       Transfer supernatant to tube. Add ethanol. White precipitate forms.

3.       Transfer suspension to syringe with filter. Push excess alcohol. Add running buffer to dissolve precipitate. Collect filtrate in tube 3.

4.       Incubate tube 3 in hot water (95°C) for 5 min. Incubate in ice water for 15 min. Drop solution on sample pad of test strip. Read results after 5-15 min.

Findings/ Result 

      If the result of Genome-Based Lateral Flow Strip Biosensor shows two lines the shrimp is positive in desease.

       If the result of Genome-Based Lateral Flow Strip Biosensor shows one line the shrimp is negative in desease.

Conclusion:

I therefore conclude that to Search easily, using the PhilGeneStrips Biosensor Kit for Quick Detection of Shrimp, and using Shrimp Farm Cloud Monitoring Mobile application, it can reads test strip result,and sends data to Shrimp Farm Cloud monitoring Website & database. In other farmers it is helpful for them because it is affordable and easily to Used.

Recommendation:

This PhilGeneStrips  Biosensor Kit For Quick Detection Of Shrimp Pathogen, The Test Strips may be coupled with PCR amplification of DNA extracts for faster, Low level Detection. PCR amplicans is detected using the Test Strip, getting rid of the electrophoresis step. They also wants to influence other people to have a knowledge on their work, and for their Application it takes time to accomplish the test result, it depends on the shrimp version.

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